电磁辐射通过转化生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：探讨强磁场应该可通过转化生长因子-β（TGF-β）-激活的视网膜-间质转换 (EMT)来促进骨髓的侵袭移至。使用增幅2Gy(60)Coγ线太阳光源自人类器官的6种骨髓，记录与EMT相关的巨大变化，这包括分别运用成像技术，抗原质斯塔夫基方规，免疫荧光技术，划痕试验车和Transwell泽田试验车来仔细观察并检验细胞组织形态，EMT标记，侵袭移至控制能力等。选用酶联免疫吸附规检验这些骨髓中所TGF-β抗原技术水平，运用特别抑制剂SB431542来审计TGF-β接收器通路在强磁场EMT中所的作用。经过增幅为2Gy太阳光的骨髓中所共存间叶细胞的表达，与；也太阳光组相比其视网膜标记更高，间叶细胞标记增高，同时其侵袭转移控制能力增强，TGF-β抗原技术水平也更高。进一步发现由A549强磁场诱导的EMT可通过对TGF-β接收器抑制发生扭转。这些结果表明TGF-β激活的EMT在强磁场诱导增强骨髓侵袭转移控制能力中所起着关键性。

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